ABSTRACT
Objective: To observe the relieving cough and reducing sputum effects of total alkaloid in Atalantia buxifolia and evaluate the safety preliminarily.Methods: The relieving cough and reducing sputum effects of total alkaloid in Atalantia buxifolia were studied by the cough model caused by the irritation of ammonia water and the phenol red output of trachea in mice.The acute toxicity test and maximum tolerance test were carried out to evaluate the safety.Results: The total alkaloid in Atalantia buxifolia at low dose could obviously prolong cough incubation period and decrease cough times in mice, and that at high dose could significantly increase the secretion of phenol red in respiratory tract, and compared with those in the blank group, the differences were statistically significant (P0.05).Conclusion: The relieving cough and reducing sputum effects of total alkaloid in Atalantia buxifolia are notable in the cough model caused by the irritation of ammonia water and the phenol red output of trachea in mice.The maximum tolerable dose test shows the total alkaloid in Atalantia buxifolia is safe.
ABSTRACT
Objective To establish an HPLC method to determine simultaneously the content of six water-soluble chemical constituents (danshensu,protocatechuic aldehyde,caffeic acid,rosmarinci acid,salvianolic acid B,and salvianolic acid A) in Xiangdan Injection,and to compare their content discrepancy of Xiangdan Injection from 20 manufacturers.Methods The HPLC method was established.The column was Alltima C18 (250 mm?4.6 mm,5 ?m) at the gradient elution mode with the flow rate of 1.0 mL/min,column temperature was kept at 25 ℃ and the detector was set at 288 nm.Results Six components were separated clearly.The relationship between the concentration and the peak areas of the six compounds was all linear.The precision,stability,repetition,and average recovery were complied with the limit.Significant difference was found in contents of six chemical constituents in Xiangdan Injections from different manufacturers that the variation of salvianolic acid A content was the most distinct and danshensu was the most quietly.Conclusion The method has been successfully used to quanlify the six compounds in Xiangdan Injection,and then,can offer the reference for the quality control of Xiangdan Injections roundly.
ABSTRACT
AIM: To establish HPLC fingerprint of Viticis Fructus. METHODS: Hypersi ODS2 column was used, with mixtures of methanol and water as mobile phase in a gradient mode. The detection wavelength was set at 270 nm . The flow rate was 1.0 mL ?min -1 . RESULTS: The RSD% of precision and reproducibility was less than 5%. CONCLUSION: The method can be used for quality control of Viticis Fructus